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. 2015 Jan 7;29(2):224–237. doi: 10.1210/me.2014-1304

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Figure 6. — DNA-demethylating drugs reduce mouse insulinoma cell proliferation and restore Meg3 expression, A and B, Cell proliferation detected by an MTT assay as an index of cell viability of MIN6-4N cells after treatment with the indicated concentrations of DNA-demethylating agents, 5′-aza-2′-deoxycytidine (DZ) and ascorbic acid (AA). An MTT assay was performed at the indicated time points. Significantly reduced number of cells was observed after 48–96 hours in all concentrations of DZ, and in 1 mM AA (P < .0001). C, Bright-field microscopy images of MIN6-4N cells showing cell death when treated with increasing concentrations of DZ and AA at the indicated time points. D, QRT-PCR assay for Meg3 using RNA isolated from MIN6-4N cells in panels A and B, showing that Meg3 expression was restored after treatment with 1 μM DZ (72 h) and 1 mM AA (96 h) (P < .0001).