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. 2014 Dec 12;29(2):274–288. doi: 10.1210/me.2014-1066

Figure 5.

Figure 5.

Selective and sustained association of MAPKs with the TNF-α gene promoter in response to glucose and IL-1β requires MAPK activity. A, ChIP analysis of selective effects of MAPK inhibitors U0126, SB203580, and SP600125 on MAPK association with the TNF-α gene promoter in MIN6 cells in response to 16.7mM glucose and 20-ng/mL IL-1β for 30 minutes. B, ChIP-qPCR time-course analysis of fold enrichment of NFATc2 and MAPKs upon the TNF-α promoter in MIN6 cells in response to IL-1β in the presence of FK506, U0126, or SB203580/SP600125 (SB/SP) relative to 2.8mM glucose controls. Graphed results are expressed as mean ± SD determined from at least 3 independent experiments. Asterisks next to plots indicate statistically significant differences (*, †, and ‡, P < .05; **, ††, and ‡‡, P < .01; ***, †††, and ‡‡‡, P < .001) in mean values for treatments compared with the stimulatory group (16.7mM glucose and 20-ng/mL IL-1β) at corresponding time points based on a one-way ANOVA and Dunnett's multiple comparison post hoc test.