FIGURE 1:

PolyIC-LMW increases CD80 on cultured human podocytes. Cultured human podocytes were stimulated with various concentrations of polyIC-LMW. FBS 1% was used as a control. (a) Total protein was extracted from the cells and processed for western blotting for human CD80. The results represent the means ± SEM (n= 3 wells) **P < 0.01; ***P < 0.001 versus 1% FBS. (b) Total RNA was extracted from the cells and processed for real-time PCR for human CD80. The results represent the means ± SEM (n= 3 wells). *P < 0.05 versus 1% FBS. GAPDH was used as an internal control.