Table 1.

Primer pairs used for quantitative RT-PCR

Gene	Sequence (F, forward; R, reverse)	Annealing temperature (°C)
Mouse
CD80	F: 5′-CGACTCGCAACCACACCATTAAG-3′	58
	R: 5′-TCCTGCCCCAAAGAGCACAAG-3′
CTLA-4	F: 5′-ACACATCTCAAGGCTTCTG-3′	53
	R: 5′-GGTCACCTGTATGGCTTC-3′
IL-10	F: 5′-GGTTGCCAAGCCTTATCG-3′	54
	R: 5′-TCTTCACCTGCTCCACTG-3′
Synaptopodin	F: 5′-TATCAACCAGAACCCGTC-3′	51
	R: 5′-AATCAAGTGTGCCATTCG-3′
β-actin	F: 5′-TCTTGGCTATGGAATCCTG-3′	60
	R: 5′-GTGTTGGCATAGAGGTCT-3′
Angiopoietin-like 4	F: 5′-GCACCAGAGCAAGTCTAA-3′	61
	R: 5′-CAGCAACGCTAGGACTCC-3′
TLR1	F: 5′-CTCATCTTCTACTGTATCATTGTT-3′	60
	R: 5′-TGGTAAGGTTTGCGTTTG -3′
TLR2	F: 5′-CCAGCAGAATCAATACAATAGA -3′	60
	R: 5′-CGAACCAGGAGGAAGATAA -3′
TLR3	F: 5′-TGGATTCTTCTGGTGTCTT-3′	60
	R: 5′-TCTTCTGAGTTGGTTGTGA-3′
TLR4	F: 5′-ATGTGATGTGACCATTGATG-3′	60
	R: 5′-ATGATTGATAAGGATTGCCATT-3′
TLR5	F: 5′-TTGGTAATATCTCCCTGTTCT-3′	60
	R: 5′-AGCCTCTCAGTGGTAGTA-3′
TLR6	F: 5′-GCATTCTTCTGGACTTAGTG-3′	60
	R: 5′-AATGGAGAACGGTGGTATT-3′
TLR7	F: 5′-GCTTTAGTGGACTCTCTGA-3′	60
	R: 5′-GTTAGATTCTCCTTCGTGATG-3′
TLR8	F: 5′-ACTCATCCATCCACATACAT-3′	60
	R: 5′-AACCAGGTAGAAGGAATCG-3′
TLR9	F: 5′-CTCTCCATACACTGAACTCT-3′	60
	R: 5′-CCTTCTTGCGGTAATTGAA-3′
TLR11	F: 5′-ACAACCAGTGTCACATCTA-3′	60
	R: 5′-GCTATAAGTCAGGCAATATCC-3′
Human
CD80	F: 5′-TTTGACCCTAAGCATCTGAAGC-3′	55
	R: 5′-ACCAGCCAGCACCAAGAG-3′
GAPDH	F: 5′-CGCTGAGTACGTCGTGGAGT-3′	64
	R: 5′-AGAGGGGGCAGAGATGATG-3′