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. 2014 Dec 17;290(6):3278–3292. doi: 10.1074/jbc.M114.622910

FIGURE 3.

FIGURE 3.

Characterization of enzymatic activity of PrsA. A, kinetics of cis-trans isomerization of 3 μm Abz-Ala-Leu-Pro-Phe-pNa followed by fluorescence at 416 nm, without enzyme (black), with 12 (blue), 16 (green), and 20 nm (red) PrsA. B, refolding kinetics of RCM-T1 in the presence of increasing concentrations of PrsA, 0 (black), 300 (blue), 500 (green), and 1000 nm (red). Catalytic efficiencies of PrsA for (C) the cis-trans isomerization of Abz-Ala-Leu-Pro-Phe-pNA and (D) the refolding of RCM-T1. The measured rate constants kapp are shown as a function of the prolyl isomerase concentration. The kcat/Km values derived from the slopes are given in Table 1.