. 2014 Dec 1;290(6):3308–3332. doi: 10.1074/jbc.M114.611525

TABLE 1.

Primers and CYP3A4 mutant constructs used in this study

Construct	Primers	Template
pCW3A4[His]₆ mutants
E283A/T284A/E285A	Sense, `GACTCTCAGAATTCAAAAGCAGCTGCGTCCCACAAAGCTCTG`	pTOPO3A4His₆
E283A/T284A/E285A	Antisense, `CAGAGCTTTGTGGGACGCAGCTGCTTTTGAATTCTGAGAGTC`	pTOPO3A4His₆
E258A/E262A/D263A	Mutagenesis carried out by GenScript (Order 328099)	pTOPO3A4His₆
E283A/T284/E285A	Sense, `CTCAGAATTCAAAAGCAACTGCGTCCCACAAAGCT`	pTOPO3A4-E283A/T284A /E285A-His₆
E283A/T284/E285A	Antisense, `AGCTTTGTGGGACGCAGTTGCTTTTGAATTCTGAG`	pTOPO3A4-E283A/T284A /E285A-His₆
E258A/E262A/D263A/S259A/T264A	Sense, `GGATGAAAGCAGCTCGCCTCGCAGCTGCACAAAAGCAC`	pTOPO3A4-E258A/E262A /D263A-His₆
E258A/E262A/D263A/S259A/T264A	Antisense, `GTGCTTTTGTGCAGCTGCGAGGCGAGCTGCTTTCATCC`	pTOPO3A4-E258A/E262A /D263A-His₆
E122A/D123A/E124A/E125A	Sense, `GCCATCTCTATAGCTGCGGCTGCAGCATGGAAGAGATTACGA`	pTOPO3A4His₆
E122A/D123A/E124A/E125A	Antisense, `TCGTAATCTCTTCCATGCTGCAGCCGCAGCTATAGAGATGGC`	pTOPO3A4His₆
S131A/S134A/T136A/S139A	Mutagenesis carried out by GenScript (Order 421880)	pTOPO3A4His₆
S259A	Sense, `CTGTAAAAAGGATGAAAGAAGCTCGCCTCGAAGATACACAAAAG`	pTOPO3A4His₆
S259A	Antisense, `CTTTTGTGTATCTTCGAGGCGAGCTTCTTTCATCCTTTTTACAG`	pTOPO3A4His₆
T264A	Sense, `GTCGCCTCGAAGATGCACAAAAGCACCGAG`	pTOPO3A4His₆
T264A	Antisense, `CTCGGTGCTTTTGTGCATCTTCGAGGCGAC`	pTOPO3A4His₆
T284A	Sense, `CTCAGAATTCAAAAGAAGCTGAGTCCCACAAAGCTCTG`	pTOPO3A4His₆
T284A	Antisense, `CAGAGCTTTGTGGGACTCAGCTTCTTTTGAATTCTGAG`	pTOPO3A4His₆
S259A/T264A	Sense, `GTCGCCTCGAAGATGCACAAAAGCACCGAG`	pTOPO3A4-S259A-His₆
S259A/T264A	Antisense, `CTCGGTGCTTTTGTGCATCTTCGAGGCGAC`	pTOPO3A4-S259A-His₆
S259A/T284A	Sense, `CTCAGAATTCAAAAGAAGCTGAGTCCCACAAAGCTCTG`	pTOPO3A4-S259A-His₆
S259A/T284A	Antisense, `CAGAGCTTTGTGGGACTCAGCTTCTTTTGAATTCTGAG`	pTOPO3A4-S259A-His₆
S478A	Ref. 37
S478D	Ref. 38
T264A/S420A/S478A	Ref. 37