TABLE 5.

CYP3A4 Lys residues found to be cross-linked to Lys residues from E2/E3 complexes

All cross-linked CYP3A4 Lys residues (except for CYP3A4K424-gp78K595) were derived from the data shown in Table 4. Residues marked with an asterisk were previously identified as residues ubiquitinated by either or both E2/E3 systems.

CYP3A4	UBC7	gp78/AMFR	UbcH5a	Hsc70	CHIP
*Lys-127			Lys-133
Lys-141	Lys-156		Lys-144
	Lys-161		Lys-4
* Lys-168	Lys-161		Lys-133
			Lys-8
Lys-209	Lys-156
	Lys-161
Lys-251	Lys-161	Lys-313
Lys-257	Lys-156	Lys-586	Lys-133		Lys-125
	Lys-161
Lys-266	Lys-161		Lys-133	Lys-512
			Lys-8
Lys-413	Lys-156
Lys-421			Lys-8		Lys-22
Lys-424		Lys-595?
*Lys-487	Lys-153
	Lys-156