TABLE 2.

Surface expression of PAR-1 and PAR-2 by flow cytometry analysis

Nonimmune mouse serum or specific monoclonal antibodies anti-PAR-1 or anti-PAR-2 were incubated with normal human epidermal melanocytes (NHEM) or melanoma cells at different stages of tumor progression, and the binding reaction of antibodies to cells was analyzed as described under “Materials and Methods.” Values represent the number of positive cells after background subtraction and are presented as mean ± S.E. (n = 3).

	% Positively staining cells
	NHEM	7GP	T1P26	c8161
Anti-PAR-1	NDa	91.8 ± 7.8	91.4 ± 5.6	91.6 ± 6.8
Anti-PAR-2	ND	28.8 ± 2.4	22.4 ± 1.6	34.6 ± 3.1

^a ND, not detected.