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. 2014 Dec 10;290(6):3455–3467. doi: 10.1074/jbc.M114.605253

FIGURE 4.

FIGURE 4.

RNAi quantification using qRT-PCR and immunoblotting. A, qRT-PCR quantification of nSMase1 KD showing successful KD in nSMase1B and nSMase1C. B, qRT-PCR quantification of nSMase2 KD showing successful KD with all lentiviruses. Due to the higher level of KD induced, nSMase2A and nSMase2B were chosen for subsequent analyses. C, immunoblot quantification of nSMase1 KD, showing a decrease for nSMase1 protein levels in nSMase1B and nSMase1C cells. Data presented as mean ± S.E., n = 3, *, p < 0.05; **, p < 0.01; ***, p < 0.001.