FIGURE 7.

Depletion of nSMase2 impairs PrP^C packaging into exosomes. A, immunoblot analyses of PrP in nSMase2A and nSMase2B cells and exosomes. B, densitometric analyses show that cellular PrP^C, PrP^Tot, and PrP^Sc levels remain unchanged. In contrast, PrP^C is decreased in non-infected nSMase2B exosomes. However, both PrP^Tot and PrP^Sc levels do not change in prion-infected exosomes. C, immunoblot analyses of Flotillin-1 and Tsg101 in nSMase2A and nSMase2B exosomes. D, densitometric analyses show that both Flotillin-1 and Tsg101 levels decrease in non-infected nSMase2B exosomes, but remain unchanged in prion-infected exosomes. Data presented as mean ± S.E., n = 3, *, p < 0.05.