TABLE 2.
Probes used to detect PDCD4 mRNA
Boldface indicates 2′-O-methyl RNA; X indicates dT-C6-NH2; all others are DNA; underlines indicate the binding region.
| Target | Ligand | Location within transcript (from start of coding region) |
|---|---|---|
| PDCD4 3′UTR 1 | 5′-Biotin-TXTTTTAAGGAGXGGCAGXCAGACAXCAAU-3′ | 1,706–1,729 |
| PDCD4 3′UTR 2 | 5′-Biotin-TXTTTTAAXAAAGCUUUGUAXAAACCAGXGA-3′ | 1,761–1,785 |
| PDCD4 3′UTR 3 | 5′-Biotin-TXTTTTGUXCCAGCCACCXUUUACUXAACUG-3′ | 1,959–1,983 |
| PDCD4 3′UTR 4 | 5′-Biotin-TXTTTTACXCCCAAXAAAGAAUCAAUACXG-3′ | 2,057–2,080 |