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. 2014 Dec 23;290(6):3508–3518. doi: 10.1074/jbc.M114.602292

FIGURE 3.

FIGURE 3.

The four C-terminal zinc-fingers of ZLD are required for DNA binding and transcriptional activity. A, -fold activation mediated by wild-type ZLD or by ZLD mutants in which individual zinc fingers in the DNA-binding domain (zinc fingers (ZF) 3–6) were mutated. Protein levels were assayed by immunoblot analysis for ZLD. B, Coomassie-stained gel of purified wild-type MBP:ZLD1117–1487 or the same protein with each of the individual zinc fingers mutated. C, electromobility shift assays using increasing concentrations of wild-type MBP:ZLD1117–1487 (0, 25, 50, 100, or 200 nm) or maximal amounts of individual zinc finger mutants (200 nm). The sequence of the probes corresponds to either a wild-type or mutant version of a CAGGTAG element from the scute promoter.