FIGURE 8.

Overexpressing GPAT1 or GPAT4 increased the protein ratio of membrane to cytosolic PKCϵ but did not alter PKC activity. Membrane and cytosolic preparations from mouse primary hepatocytes overexpressing EGFP, Flag-GPAT1, Flag-GPAT3, or Flag-GPAT4 were subjected to Western blotting (A) and protein quantification (B) or assayed for PKC activity (C). A and B show representative Western blots and protein quantifications from three independent experiments; C shows data from three independent experiments, each done in triplicate. *, p < 0.05 compared with EGFP.