FIGURE 3.

Salinomycin prevents TGFβ-induced collagen production in primary human fibroblasts. A, primary human fibroblasts were treated with salinomycin (10–250 nm) and TGFβ (1 ng/ml) for 72 h, and then cell culture supernatants were collected and analyzed for collagen I levels by slot blot analysis. Salinomycin reduced collagen I levels in a dose-dependent manner, with salinomycin (100 and 250 nm) reducing collagen production to baseline levels. B, fibroblasts were treated with 250 nm salinomycin and TGFβ (1 ng/ml), and cell culture supernatants were collected at 5, 24, 48, and 72 h to analyze production of collagen I. Salinomycin prevented collagen production at 24, 48, and 72 h. Experiments were performed in at least three primary human fibroblast strains, with representative strains shown.