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RTX dominates the human immune response to ACT. Nine serum samples from humans exposed to B. pertussis were tested for reactivity to the catalytic domain, RTX751, or intact ACT by ELISA. Absorbance values at a 100-fold dilution of the sera were normalized to that of ACT at the same dilution. A paired t test was used to determine the statistical significance between signals for CAT and RTX binding domains. ***, p ≤ 0.001.
