. 2014 Dec 10;290(6):3576–3591. doi: 10.1074/jbc.M114.585281

TABLE 2.

Biochemical analysis of ACT constructs

Binding of previously characterized anti-ACT murine antibodies (47) to ACT and various derivatives described in this work in ELISAs. ACT constructs were coated on ELISA plates, and thee antibodies were titrated and detected with anti-mouse IgG-HRP.

ACT domain	3D1 epitope 373–399	2A12 epitope 399–828	10A1 epitope 624–780	2B12 epitope 888–1006	6E1 epitope 1320–1489	9D4 epitope 1156–1489	7C7 epitope 1320–1627	1H6 epitope 1590–1706	10A8 epitope 1590–1706
CAT₄₀₀	+++
HP₁₀₉₆		+++	+
HP₁₀₉₆*		+++	+
RTX₉₈₅					++	++	++	+++	+++
RTX₇₅₁				+	++	++	+	+++	+++
RTX₇₅₁*				+	++	++	+	+++	+++
ACT	+++	+++	++	++	+++	+++	+	++	+++