FIGURE 10.
A bootstrapped tree of the Csd4 family of CPs. Homologs of Csd4 were identified from the nonredundant database at the National Center for Biotechnology Information utilizing BLASTP and an E value cutoff of 1 × 10−7. Identical protein sequences derived from different strains of the same species and proteins with short alignment coverage were removed. The sequences were aligned with Clustal Omega (28) and a bootstrapped tree (with 500 replicates, subtree pruning and regrafting and five random starts) was generated using PhyML (29) within Seaview (30). *, H. hepaticus and H. cinaedi form their own subbranch and contain a His at the equivalent position of Gln-46.