FIGURE 10.

Effect of ions and dehydration on the dephosphorylation rate. Pig kidney enzyme was phosphorylated as described under “Experimental Procedures.” Phosphorylated enzyme was diluted for the indicated time intervals in media containing K⁺, Acet⁺, or Form⁺, producing a final cation concentration of 1 mm (open circles), as indicated. Closed circles indicate dephosphorylation of the enzyme as above, but with diluting solution containing glucose, producing a final concentration of 15%. Data were analyzed using a monoexponential decay function, giving the indicated rate constants of phosphoenzyme hydrolysis for control (K_Cnt) or in the presence of glucose (K_Glc).