FIGURE 3.
Phylogenetic analysis and sequence alignment of PsbA4 from Cyanothece 51142. A, phylogenetic tree showing newly identified sD1 homologs across 130 cyanobacteria with sequenced genomes. sD1 and normal D1 sequences are highlighted in pink and green boxes, respectively. The scale bar indicates number of amino acid substitutions per site. B, amino acid sequence alignment of the C-terminal regions of sD1 protein from selected cyanobacterial species (using CLUSTAL OMEGA). Alignment illustrates the variability of amino acid sequence after Ala-344 between the different sD1 homologs. Conserved residues are highlighted in bold. C, alignment of amino acid sequences for PsbA2 from Synechocystis 6803 and all five c-PsbAs from Cyanothece 51142. C-terminal regions are shown to indicate the CtpA recognition sequences (gray-shaded area). The amino acid replacements in c-PsbA4 are highlighted in red.