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. 2014 Dec 22;290(6):3784–3792. doi: 10.1074/jbc.M114.605287

FIGURE 4.

FIGURE 4.

Genetic loss of ARIA reduces atherosclerosis. A, en face preparation of aorta stained with oil red-O (ORO). ARIA and ApoE DKO mice showed significantly reduced atherosclerotic lesion. **, p < 0.01 (n = 10 each). Bar: 5 mm. B, histology of plaques at the aortic sinus stained with oil red-O. Oil red-O-positive lipid-rich lesion was significantly reduced in the plaques of DKO mice. **, p < 0.01 (n = 13 each). Bar: 100 μm. C, histology of plaques at the aortic sinus immunostained with anti-CD68 antibody. Macrophage content in plaques at the aortic sinus was not different between ApoE−/− and DKO mice. #, NS (n = 5 each). Bar: 100 μm. D, histology of plaques at the aortic sinus stained with Masson's trichrome. Collagen fibers were significantly increased in the plaques of DKO mice. *, p < 0.05 (n = 15 each). Bar: 100 μm. E, histology of plaques at the aortic sinus stained with hematoxylin and eosin. Necrotic core was significantly reduced in the plaques of DKO mice. *, p < 0.05 (n = 10 each). Bar: 100 μm. F, serum lipid profiling of ApoE−/− or DKO mice fed a high cholesterol-diet for 15 weeks. Levels of serum cholesterol and triglycerides were similar between ApoE−/− and DKO mice (n = 10 each). G, foam cell formation of resident PMs isolated from ApoE−/− or DKO fed an HCD. Resident PMs from DKO mice fed an HCD showed significantly reduced foam cell formation. **, p < 0.01 (n = 10 each). Error bars in all panels indicate mean ± S.E.