FIGURE 2.

Exonuclease kinetics. Preformed enzyme-DNA complexes were rapidly mixed with Mg²⁺. The loss of primer was plotted as a function of time and fit to a biphasic decay equation to obtain rate constants for the fast phase. The rate constants obtained are given in Table 2. A and B, exonucleolytic degradation of DNA substrates by Pol2_core and Pol ϵ, respectively. Shown are single-stranded DNA (51T) (yellow, ▴), correctly primed template (50/80) (black, ▾), and single mismatch at the primer end (51T/80) (red, ■). Green lines (●), exonucleolytic degradation of double mismatch at the primer end (51TT/80). Error bars, S.E.