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Elemental effect. Single-turnover nucleotide incorporation reactions were performed with Pol2core exo− (A) and Pol ϵ exo− (B) either in the presence of 15 μm dTTP (black line, ●) or in the presence of 15 μm (Sp)-dTTPαS (red line, ■). The amount of product formed is plotted against time and fit in a biphasic association equation. Mean values of the two independent experiments are plotted.
