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Kinetic parameters obtained by global fitting of processive polymerization
| Nucleotide | Rates of polymerization |
Dissociation rates |
||
|---|---|---|---|---|
| Pol2core exo− | Pol ϵ exo− | Pol2core exo− | Pol ϵ exo− | |
| s−1 | s−1 | |||
| 1st | 54 ± 1 | 92 ± 1 | 0.8 ± 0.48 | 1.00 ± 0.12 |
| 2nd | 151 ± 3 | 174 ± 4 | 4.4 ± 1.3 | 1.58 ± 0.20 |
| 3rd | 142 ± 5 | 175 ± 7 | 4.4 ± 0.39 | 2.04 ± 0.04 |
| 4th | 251 ± 35 | 345 ± 92 | 3.3 ± 0.29 | 3.79 ± 3.00 |
| 5th | 162 ± 4 | 275 ± 11 | 6.3 ± 0.50 | 13 ± 7.5 |
