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. 2015 Feb 6;5:8293. doi: 10.1038/srep08293

Figure 2. WDR5 promotes bladder cancer cell proliferation in vitro.

Figure 2

(A) Efficiency of WDR5 knockdown in UM-UC-3 cells by siRNA was verified by qRT-PCR and Western blotting. (B) Efficiency of WDR5 stable overexpression in UM-UC-3 and T24 cells by lentivirus was verified by qRT-PCR and Western blotting. The samples were derived from the same experiment and that blots were processed in parallel. The full-length blots are presented in Supplementary Figure 4. (C and D) Influence of WDR5 knockdown or overexpression on viability of UM-UC-3 and T24 cells was measured by MTT assay. (E and F) Effect of WDR5 knockdown or overexpression on colony formation was measured in UM-UC-3 and T24 cells. Representative images of the colonies, stained with crystal violet, were shown above, whereas the colony counts were shown below the graph. Three independent experiments yielded similar results. The results are presented as the means ± SD of values obtained in three independent experiments. Statistical significance was calculated using the Student's t-tests when only two groups were compared or ANOVA when more than two groups were compared. *p < 0.05, **p < 0.01.