Figure 3. WDR5 knockdown induced G0/G1 phase cell cycle arrest in bladder cancer cells.

(A) UM-UC-3 and T24 cells were transfected with WDR5 or control siRNA for 48 h and analyzed by flow cytometry. Percentages (%) of cell populations at different stages of cell cycles are listed within the panels. All histograms show the percentage (%) of cell populations from three independent experiments. (B) The cell population at S phase was measured by EdU assay. Blue color represents the nucleus and red color indicates S phase cells (EdU-positive). (C) Histological analysis of the percentage of EdU-positive cells in control and WDR5 knockdown. (D) Effect of WDR5 knockdown on regulation of cyclins was detected by Western blotting. WDR5 silencing reduced the expression of cyclin B1, cyclin E1 and cyclin E2. The samples were derived from the same experiment and that blots were processed in parallel. The full-length blots are presented in Supplementary Figure 4.The results are presented as the means ± SD of values obtained in three independent experiments. Statistical significance was calculated using the ANOVA. *p < 0.05, **p < 0.01.