Figure 5. WDR5 enhances bladder cancer cell chemoresistance to cisplatin.

(A) The cell viability of cells transfected with WDR5 or control siRNA and treated with cisplatin for 48 h was analyzed by MTT assay. (B) The cell viability of cells that stably overexpressed WDR5 or control vector and treated with cisplatin for 48 h was analyzed by MTT assay. For calculation of IC₅₀, four parameter logistic curve (best-fit solution, nonlinear regressiondynamic fitting) and normality tests are used (Graph Pad Prism 5). (C) The cells 24 h after transfection with control or WDR5 siRNA were treated with 0 or 2 μg/ml cisplatin for 24 h. The percentage of apoptotic cells was analyzed by flow cytometer. (D) The histogram showed the percentage (%) of late and early apoptotic cells from three independent experiments. (E) Caspase 3/7 activity assay was performed on the UM-UC-3 and T24 cells transfected with control or WDR5 siRNA and treated with or without IC₅₀ concentration of parental cells cisplatin for 24 h. The UM-UC-3 cells were treated with 1.47 μg/ml, while the T24 cells were treated with 1.82 μg/ml. Relative caspase 3/7 activity is indicated as percentage of untreated parental cells. The results are presented as the means ± SD of values obtained in three independent experiments. Statistical significance was calculated using the Student's t-tests when only two groups were compared or ANOVA tests when more than two groups were compared. *p < 0.05, **p < 0.01.