Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 30;14:133. doi: 10.1186/s12935-014-0133-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Hanif et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Effect of NA-2 and TMZ alone and in combination on active caspase-3 expression. (A) Representative Photomicrographs of cleaved caspase-3 immunoreactivity in control (DMSO treated) and treated group of U87 cells. Base line immunofluorescence was detected in control group. Significant Increase in immunofluorescence is detected in all treated groups with the highest observed intensity in combination treatment group (NA-2 + TMZ). (B) Quantification of cleaved caspase-3 expression using Image J software, cells were counted in the field then percent intensity (arbitrary unit) was calculated. Each bar represents mean ± S.E.M of three independent experiments. Significant difference between vehicle control and treated cells was indicated ***P <0.001. The significant difference between the individual drugs NA-2 or TMZ and their combination treatment is indicated by ^$$ P <0.01, and ^### P <0.001 respectively.