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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: Nat Neurosci. 2013 Jul 14;16(8):1016–1023. doi: 10.1038/nn.3458

Fig. 2. Complementary fate maps reveal the distribution of r-derived norepinephrine subpopulations in the pontine norepinephrine nuclei.

Fig. 2

(a) Coronal sections from adult mouse brainstems reveal the contribution of r1(En1cre;DbhFlpo;RC∷FrePe)-, r2(Hoxa2-cre;DbhFlpo;RC∷FrePe)-, r3&5(Krox20cre;DbhFlpo;RC∷FrePe)-, and r4(Hoxb1cre;DbhFlpo;RC∷FrePe)-derived norepinephrine neurons to the SubCV, LoC, and A5 nuclei. eGFP (green) marks the r-derived norepinephrine population and mCherry (red) marks all other norepinephrine neurons in the representative sections corresponding to the boxed areas within the schematics (left). Scale bar indicates 200 μm (LoC) and 166 μm (SubCV and A5). (b) Cell counts of eGFP and mCherry positive neurons reveal the contribution of norepinephrine neurons derived from r1, r2, r3&5 and r4 to the LoC, SubCD, SubCV, A7, and A5 pontine nuclei. Numbers are the sum of bilateral counts from 30 μm sections spaced 120 μm apart from the brainstem of four animals for each fate map (error bars are mean ± s.e.m). Green bars represent counts of eGFP positive r-derived norepinephrine neurons, and red bars represent the counts of mCherry positive norepinephrine neurons (all other non-r-derived norepinephrine neurons). Total cell counts (mCherry plus eGFP positive cells) for each norepinephrine nucleus were not significantly different between fate maps (One-way ANOVA analysis p > 0.05; df=3 and 12; LoC F=0.6073; SubCD F=2.727; SubCV F=2.203; A7 F=0.7999; A5 F=2.694).