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The range of time scales over which signaling events occur and their reporters’ function. The response time within each class of reporter may vary; for example, a transcriptional reporter expressing superfolder GFP (sfGFP) will be detectable before one expressing GFP. Knockdown experiments or genetic knockouts may take many hours or days to take effect during which time secondary effects will occur. Credit: V. ALTOUNIAN/SCIENCE SIGNALING
