Table 1.

BrM lipid composition: studies using tissues.

Reference	N	Age, yr {1}	D to P, hr {2}	Fresh/fixed	Region	Tissues	Prep	Assays
(Sheraidah et al., 1993)	27	1.5, 97	n.a.	Fixed 24 h	M	RPE/BrM/Ch, BrM/Ch	Cryosections	TLC; GC, FID
(Holz et al., 1994)	32	3, 97	17.6	Fresh	M, P	BrM/Ch	7 mm	TLC
(Gülcan et al., 1993)	12 pr	4th, 9th decade	<24	Fixed 1 h	M, P	Ret; RPE; BrM/Ch	4 mm	HPLC; saponification & HPLC for FA
(Pauleikhoff et al., 1994)	27	1, 97	n.a.	Fixed 24 h	M	RPE/BrM/Ch	Cryosections	TLC; GC, FID
(Spaide et al., 1999)	13	40, 78	<24	Fresh	M	BrM/Ch	7 mm; vessels removed; histo	Saponified; HPLC for FA
(Curcio et al., 2001)	10	71, 87	<4	Fixed, various times	M (10), P (3)	Ret; BrM/Ch; Ch vessels	8 mm; vessels removed; histo	Enzymatic fluorimetric assay *
(Li et al., 2005a,b)	2	>60	<6	Fresh	All	Ret; BrM	8 mm; vessels removed; histo	ESI/MS
(Bretillon et al., 2008a,b)	27	59, 95	34	Fresh	All	Ret; RPE/Ch	Entire retina, entire RPE/Ch	TLC; GC, FID; saponification & HPLC for FA in retina PL
(Wang et al., 2009a,b)	4	37, 86	<8	Fresh	All	BrM/Ch	Entire BrM, vessels removed	TLC; GC, FID

Notes and abbreviations (see also table at beginning of article).

Age: Minimum and maximum age.

D to P: Death to preparation time.

Fresh/fixed: fixatives were formalin or 4% paraformaldehyde.

Tissues: Ch, choroid.

Region: M, macula; P, periphery.

Prep: Diameter of circular punch given; Histo, quality of dissection monitored by histology.

Assays: ESI/MS, electrospray ionization mass spectrometry; FID, flame ionization detection of fatty acids; GC, gas chromatography; HPLC, high performance liquid chromatography; TLC, thin layer chromatography.

n.a., not available.

^*EC mass was originally reported as nmol/g dry weight and should be (nmol × 1000)/g dry weight.