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. 2014 Dec 22;125(2):551–562. doi: 10.1172/JCI78470

Figure 1. WNT/mTORC1-dependent protein synthesis induces a transient energy deficit during osteoblast differentiation.

Figure 1

(A) Effect of Torin1 on WNT-induced mTORC1 signaling and AMPK phosphorylation assayed by Western blot. Phospho-proteins normalized to respective total protein. Fold change ± SD for WNT3A over vehicle in 3 independent experiments. (B and C) Metabolic labeling of protein synthesis in response to WNT3A (B) and the effect of Torin1 (C). Cyclohexamide (Chx) used to inhibit protein synthesis as a control in B. Error bars indicate SD. *P < 0.05, Student’s t test. n = 3. (D) Effect of WNT3A on AMPK phosphorylation at 1, 6, and 12 hours. Fold change ± SD from 3 independent experiments.