(A–D) Effect of BPTES treatment on WNT-induced Gln consumption (A), gene expression (B and C), or AKP2 and von Kossa staining (D). (E–H) Effect of DM-α-KG on WNT-induced Gln consumption (E), GCN2 phosphorylation (F), anabolic gene expression (G), or AKP2 and von Kossa staining (H). Error bars indicate SD. *P < 0.05, Student’s t test. n = 3.