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. 2015 Jan 9;125(2):652–664. doi: 10.1172/JCI77701

Figure 6. Expression of hydroxylation- and degradation-resistant HIF2α-mutant HIF2α-DPA in HLMVECs stabilizes the endothelial barrier via VE-PTP expression.

Figure 6

(A) HLMVECs were infected with lentivirus expressing a Flag-tagged WT HIF2α or with a hydroxylation- and degradation-resistant HIF2α-DPA mutant. VE-PTP and VE-cadherin protein expression was determined by WB. Quantification of VE-PTP (B) and VE-cadherin (C) expression (n = 3/group). (D and E) HLMVECs infected with lentivirus expressing WT, a HA-HIF1α-DPA mutant, or a Flag-HIF2α-DPA mutant were immunostained for VE-cadherin and analyzed by confocal microscopy. Scale bars: 20 μm. Blot images were derived from samples run on parallel gels. ***P < 0.005, ****P < 0.001 by Student’s t test.