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. 2015 Jan 20;125(2):699–714. doi: 10.1172/JCI76210

Figure 6. CNS tissue does not mediate the effects on CD4+ T cells through antigen or IL-4.

Figure 6

(A) Gata3 expression in sorted wild-type CD4+ T cells cocultured with MHCII KO antigen-presenting cells in the presence or absence of optic nerves from uninjured mice (n = 3 per group; representative of 2 experiments). (B) Gata3 expression in sorted OTII/Rag1–/– CD4+ T cells cocultured with wild-type antigen-presenting cells in the presence or absence of optic nerves from uninjured mice (n = 9 wild-type; n = 5 OTII/Rag1–/–; representative of 2 experiments). (C) Gata3 expression in sorted wild-type CD4+ T cell cultures lacking antigen-­presenting cells incubated in the presence or absence of optic nerves from uninjured mice (n = 3 per group; representative of >3 experiments). (D) Gata3 expression in sorted CD4+ T cells after culture in the presence or absence of isolated optic nerves placed in a Transwell with 0.4-μm pores (n = 3 per group; representative of 2 experiments). (E) Gata3 expression in sorted CD4+ T cells after culture in optic nerve–conditioned media or control media (n = 5 per group; representative of 2 experiments). (F) Gata3 expression (mean ± SEM) in sorted CD4+ T cells after culture in the presence or absence of isolated optic nerves from Il4–/– mice (n = 4 per group; representative of 2 experiments). *P < 0.05; **P < 0.01; ***P < 0.001, 2-tailed Student’s t test used for statistical analysis. Error bars represent SEM.