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. 2014 Dec 22;125(2):539–550. doi: 10.1172/JCI76887

Figure 5. RAGE, but not TLR4, mediates neutrophil recruitment following necrosis.

Figure 5

(A) Migration of WT, Tlr4–/–, and RAGE-deficient (RAGE encoded by Ager) neutrophils (Ager–/– neutrophils, n ≥2 separate isolations per experiment) toward WT liver lysates. GM-CSF served as a positive control. (B–E) Analysis of hepatic neutrophil infiltration (B), inflammatory gene expression (C), H&E staining and necrosis quantification (D), and serum ALT concentrations (E) in WT (n = 9) and Tlr4–/– (n = 7) mice treated with acetaminophen (300 mg/kg). (F–I) Analysis of hepatic neutrophil infiltration (F), inflammatory gene expression (G), H&E staining and necrosis quantification (H), and serum ALT concentrations (I) in WT (n = 10) and Ager–/– (n = 9) mice treated with acetaminophen (300 mg/kg). *P < 0.05 and **P < 0.01 by unpaired 2-tailed t test. Scale bars: 200 μm.