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. 2014 Dec 22;125(2):539–550. doi: 10.1172/JCI76887

Figure 6. RAGE-expressing neutrophils promote injury amplification after necrotic injury.

Figure 6

(A–D) Neutrophil infiltration (A), inflammatory gene expression (B), H&E staining and necrosis (C), and serum ALT concentrations (D) in chimeric mice with WT BM (n = 8) and Ager–/– BM (n = 10) treated with acetaminophen (300 mg/kg). (E–I) Chimeric mice with WT BM and Elane–/– BM (n = 12 per group) were treated with acetaminophen (300 mg/kg). Hepatic neutrophil infiltration was determined by staining for Ly6-B (E). BM reconstitution was confirmed by qPCR for Elane in spleens of BM-chimeric mice (F). Hepatic inflammatory gene expression was determined by qPCR (G). Liver injury was assessed by hepatic H&E staining and necrosis quantification (H), and serum ALT concentrations (I). *P < 0.05 and **P < 0.01 by unpaired 2-tailed t test. Scale bars: 200 μm.