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. 2015 Jan 2;125(2):593–606. doi: 10.1172/JCI77780

Figure 5. Specific colocalization of NY-ESO-1 peptide and MHC class II in NY-ESO-1–overexpressing glioma cell line LN229 in vitro.

Figure 5

(A) IF of LN229 cells stably overexpressing myc-tagged NY-ESO-1 (LN229 NY). Green, NY-ESO-1; red, myc; blue, DAPI. Scale bar: 20 μm. (B and C) NY-ESO-1-HLA-DR PLA on (B) NY-ESO-1–overexpressing LN229 cells and (C) empty vector control cells (LN229 EV) costained with anti-myc. Red, PLA signal; green, myc; blue, DAPI. Scale bar: 20 μm. Data are representative of 3 experiments each.