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. 2015 Jan 20;125(2):846–858. doi: 10.1172/JCI73688

Figure 2. Effects of the anti-ghrelin compound.

Figure 2

(A) Representative images of ghrelin-induced cFos IR (marker of cellular activation) from P12 and P36 mice neonatally injected with control or anti-ghrelin. (B) Quantitative comparisons of ghrelin-induced cFos IR in the ARH of P12 mice 2, 6, 12, and 24 hours after i.p. administration of control or anti-ghrelin (n = 3 for control; n = 4 for saline and anti-ghrelin). The gray bar shows the number of cFos-IR cells in saline-treated animals. (C) Number of cFos-IR cells of P21 mice neonatally injected with control or anti-ghrelin 2 hours after i.p. administration of ghrelin (2 mg/kg) (n = 3 per group). (D) Stomach content of P14 pups injected with the control or anti-ghrelin compound (n = 7 for control; n = 8 for anti-ghrelin). (E) Relative expression of ghrelin mRNA in the stomachs of P14 pups injected with control or anti-ghrelin (n = 7 per group). (F) Total plasma ghrelin levels of P14 pups injected with control or anti-ghrelin (n = 7 per group). Values are shown as the mean ± SEM. *P < 0.05 vs. control. Statistical significance was determined using 2-tailed Student’s t tests (C–F) and a 2-way ANOVA followed by Bonferroni’s post-hoc test (B). Scale bar: 120 μm.