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. 2015 Jan 20;125(2):846–858. doi: 10.1172/JCI73688

Figure 6. Chronic neonatal hyperghrelinemia causes metabolic disturbances.

Figure 6

(A) Schematic representation of the experimental design used to increase ghrelin levels during neonatal life. Starting at P4, pups were treated daily with i.p. injections of ghrelin (2 mg/kg) (PN ghrelin) or vehicle (control), for a total of 8 days. (B) Acylated ghrelin levels in P10 mice injected with vehicle (0.9% NaCl) or ghrelin (2 mg/kg) (n = 4 for vehicle; n = 6 for PN ghrelin). Values are shown as the mean ± SEM. *P < 0.05 vs. control. (C) Pre- and postweaning growth curves (body weights) of mice neonatally injected with control or ghrelin (n = 5 for vehicle; n = 7 for PN ghrelin). (D) Plasma leptin and (E) insulin levels at 90 days of age in mice neonatally injected with control or ghrelin (n = 4 for control; n = 5 for PN ghrelin). (F) Fasting glucose levels in P80 mice neonatally injected with control or ghrelin (n = 5 for control; n = 7 for PN ghrelin). Values are shown as the mean ± SEM. *P < 0.05 vs. vehicle. Statistical significance was determined using 2-tailed Student’s t tests (D–F) and a 2-way ANOVA followed by Bonferroni’s post-hoc test (B and C).