Figure 2. Studies of the Astro-WSB-1KO mouse.

(A) Scheme of the mouse Wsb1 gene showing the 2 LoxP sites flanking exons 6 and 7. When the Wsb1^fl/fl mouse is crossed with the GFAP-Cre mouse, exons 6 and 7 are floxed out and a frame stop codon is formed in exon 8 that prevents translation of the SOCS box located in exon 9. (B) Wsb1 mRNA levels in the hippocampus, cerebellum, cerebral cortex, and hypothalamus of Astro-WSB-1KO and littermate control mice. (C) Same as in B, except that Dio2 mRNA levels are shown. (D) Same as in C, except that D2 activity is shown. (B–D) Results are expressed as mean ± SD of 5–6 animals per group. *P < 0.01 vs. GFAP-Cre littermate control mice.