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. 2015 Jan 9;125(2):665–680. doi: 10.1172/JCI78253

Figure 7. Deficiency of FASN suppresses the transcription of Nlrp3 and Il1b genes through p38 MAPK in macrophages.

Figure 7

(A) Quantitative PCR analysis for Nlrp3, Il1b, Il18, Tnf, and Il12 gene expression in wild-type mouse peritoneal macrophages transduced with lentiviruses expressing nontarget shRNA or shRNA for FASN (shFASN) and stimulated with LPS (500 ng/ml) for 4 hours. **P < 0.01, ANOVA. (B) Immunoblot analysis for activation of p38 MAPK, ERK, and JNK in cell lysates from wild-type mouse peritoneal macrophages transduced with control shRNA or shRNA for FASN and stimulated with LPS (500 ng/ml) for 0, 10, 20, and 30 minutes.