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. 2015 Jan 9;125(2):665–680. doi: 10.1172/JCI78253

Figure 9. Inhibition of p38 MAPK activation suppresses the transcription of Nlrp3 and Il1b genes in macrophages.

Figure 9

(A) Quantitative PCR analysis for Nlrp3, Il1b, Il18, and Tnf gene expression in cell lysates and (B) immunoblot analysis for expression of NLRP3 and IL-1β in cell lysates from wild-type BMDMs pretreated with SB203580 (10 μM), U0126 (10 μM), and SP600125 (10 μM) for 1 hour before stimulation with LPS (500 ng/ml) for 4 hours. *P < 0.05, **P < 0.01, ANOVA. β-Actin served as the standard.