. 2015 Feb 6;6:55. doi: 10.3389/fmicb.2015.00055

Table 2.

Anaplasma phagocytophilum proteins identified in the nuclear lysates of infected HL-60 cells by iTRAQ with ratios compared with uninfected cells of >1.2 and ranked by Unused ProtScore to identify high likelihood candidates for nuclear translocation.

Locus name	Accession	Protein	Gene	Ratios of labeled peptides^a			Mean HL-60	Mean Aph	Ratio Aph:HL-60	Unused ProtScore
				115:114	116:114	117:114
APH_0740	gi\|88607707	Ankyrin A	ankA	1.06	1.42	1.43	1.03	1.43	1.38	40.10
APH_1023	gi\|88607105	DNA-directed RNA polymerase subunit beta; RNAP subunit beta	rpoC	1.04	1.50	1.39	1.02	1.45	1.42	32.10
APH_0240	gi\|88606723	60 kDa chaperonin GroEL	groEL	1.00	1.88	1.93	1.00	1.90	1.90	30.60
APH_1024²	gi\|88606872	DNA-directed RNA polymerase subunit beta; RNAP subunit beta	rpoB	1.02	1.33	1.27	1.01	1.30	1.28	23.40
APH_0906	gi\|88606911	Hypothetical protein APH_0906		1.05	1.25	1.22	1.02	1.24	1.21	20.70
APH_0278²	gi\|88607578	Translation elongation factor Tu; EF-Tu	tuf1	1.18	1.93	1.83	1.09	1.88	1.73	20.20
APH_1099	gi\|88607685	DNA-binding response regulator CtrA	ctrA	1.05	2.65	2.71	1.03	2.68	2.62	19.90
APH_0303	gi\|88606699	DNA-directed RNA polymerase subunit alpha; RNAP subunit alpha	rpoA	1.16	1.91	1.84	1.08	1.88	1.74	15.90
APH_0784	gi\|88606926	DNA-binding protein HU	hup	1.00	2.38	2.03	1.00	2.21	2.21	15.40
APH_0968	gi\|88606840	ATP-dependent protease La	lon	1.01	1.62	1.44	1.01	1.53	1.52	15.10
APH_1100	gi\|88606714	DNA-binding protein		0.99	2.82	2.44	0.99	2.63	2.64	13.80
APH_0469	gi\|88607025	Putative malonyl-CoA decarboxylase		1.04	1.27	1.28	1.02	1.27	1.24	12.60
APH_0445	gi\|88607683	Transcription elongation factor NusA	nusA	1.00	1.53	1.50	1.00	1.52	1.52	12.20
APH_0339	gi\|88607311	Putative thermostable metallocarboxypeptidase		1.11	1.59	1.58	1.05	1.58	1.50	9.60
APH_1239	gi\|88607921	P44–15b outer membrane protein; major surface protein-2C	p44–15b	1.05	3.60	3.63	1.03	3.62	3.53	9.10
APH_0062	gi\|88606901	Hypothetical protein APH_0062		1.06	1.91	1.77	1.03	1.84	1.79	8.70
APH_1097	gi\|88607712	DNA polymerase III, beta subunit	dnaN	1.14	1.33	1.30	1.07	1.32	1.23	6.60
APH_0135	gi\|88606701	Cold shock protein, CSD family		0.97	1.79	1.78	0.99	1.79	1.81	6.30
APH_0397	gi\|88606909	30S ribosomal protein S2	rpsB	1.07	1.53	1.45	1.04	1.49	1.44	6.20
APH_1263	gi\|88607227	Translation initiation factor IF-3	infC	0.94	2.12	1.97	0.97	2.05	2.11	5.00
APH_0398	gi\|88607503	Elongation factor Ts; EF-Ts	tsf	1.03	1.24	1.28	1.01	1.26	1.24	4.40
APH_1151	gi\|88607101	Hypothetical protein APH_1151		1.20	2.16	2.11	1.10	2.13	1.94	4.10
APH_0288	gi\|88607038	50S ribosomal protein L29	rpmC	1.03	1.77	1.66	1.01	1.72	1.69	4.10
APH_1029	gi\|88607731	Transcription termination/antitermination factor NusG	nusG	0.94	1.65	1.72	0.97	1.69	1.74	4.00
APH_1027	gi\|88607420	50S ribosomal protein L1	rplA	1.15	1.37	1.26	1.08	1.31	1.22	3.30
APH_0515	gi\|88606905	Expression regulator ApxR	apxR	0.99	2.02	1.94	0.99	1.98	2.00	3.20
APH_0097	gi\|88606982	Protein-export protein SecB	secB	1.11	1.81	1.67	1.06	1.74	1.64	3.00
APH_0292	gi\|88606711	50S ribosomal protein L5	rplE	1.26	1.52	1.57	1.13	1.54	1.36	2.40
APH_0106	gi\|88607568	Riboflavin synthase, alpha subunit	ribE	0.98	2.04	2.00	0.99	2.02	2.04	2.30
APH_0280	gi\|88607449	50S ribosomal protein L3	rplC	1.07	1.60	1.64	1.04	1.62	1.56	2.30
APH_0629	gi\|88607793	Malate dehydrogenase	mdh	0.98	1.25	1.21	0.99	1.23	1.24	2.30
APH_0160²	gi\|88606875	Putative thymidylate synthase, flavin-dependent, truncation		1.14	1.50	1.37	1.07	1.44	1.34	2.20
APH_0154	gi\|88607134	Serine hydroxymethyltransferase SHMT	glyA	1.06	1.29	1.26	1.03	1.27	1.24	2.10
APH_0971	gi\|88607838	Trigger factor; TF	tig	1.04	1.37	1.28	1.02	1.32	1.30	2.00
APH_0659	gi\|88607183	Antioxidant, AhpC/Tsa family		0.99	1.26	1.23	1.00	1.24	1.25	2.00
APH_1349	gi\|88606948	Glyceraldehyde-3-phosphate dehydrogenase, type I	gap	0.86	1.13	1.19	0.93	1.16	1.24	2.00
APH_1198	gi\|88606994	2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase	sucB	0.98	1.24	1.16	0.99	1.20	1.21	2.00
APH_1025	gi\|88607605	50S ribosomal protein L7/L12	rplL	1.01	1.85	1.79	1.01	1.82	1.81	1.90
APH_0289²	gi\|88607574	30S ribosomal protein S17	rpsQ	0.90	1.57	1.46	0.95	1.52	1.60	1.70
APH_1034²	gi\|88607212	30S ribosomal protein S7	rpsG	1.16	1.42	1.41	1.08	1.41	1.31	1.50
APH_0196²	gi\|88607673	Response Regulator NtrX, putative nitrogen assimilation regulatory protein	ntrx	0.93	1.70	1.50	0.97	1.60	1.66	1.40
APH_1333²	gi\|88607617	Transcription elongation factor GreA	greA	0.95	1.29	1.27	0.98	1.28	1.31	1.30
APH_1098	gi\|88607131	3′–5′ exonuclease family protein		1.12	1.30	1.29	1.06	1.30	1.22	1.30

Isobaric ion labels of nuclear lysates from: 114 and 115, uninfected HL-60 cells; 116 and 117, A. phagocytophilum-infected HL-60 cells.

^b Not cloned.