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. 2015 Feb 6;6:29. doi: 10.3389/fmicb.2015.00029

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Copyright © 2015 Wilson, Xiang and Plebanski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dendritic cell activation in dLNs after injection with PSNPs, Montanide, and Poly I:C. Mice (BALB/c) were injected once intradermally at the base of tail with the different adjuvants alone. 48 h after injection, mice were sacrificed, local (inguinal) dLNs were harvested and the levels of CD11c⁺ DCs and various activation markers were assessed by flow cytometry. (A) gating strategy; (B) frequency of GR-1^-CD11c⁺ cells; (C) Mean fluorescent intensity (MFI) of CD80 and CD86 on GR-1^-CD11c⁺ cells; (D) MFI of CD40 and MHCII on GR-1^-CD11c⁺ cells. Data presented as mean ± SD of MFI for each group of treatment (n = 3 mice/group). Statistical analysis was performed via t-tests, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.