Table 2.
Primers and DNA oligonucleotide used in this study
| Target | Forward primer | Reverse primer | |
|---|---|---|---|
| Primer pair | |||
| 1 | patA | TCTTGCTCAGTCCATCATCGAA-TATA | CCGCTGTGGATTAGTTCATTTCC |
| 2 | patB | AGAATCCAGTCCAGCGAAAGCT | GAAAGAACGACCAGATGTTCCAAT |
| 3 | patA first half | TCTTGCTCAGTCCATCATCGAA-TATA | CAGCATCGGTTCCTTGTC |
| 4 | patA second half | CAGATGAAGAGTTGGTTGGA | CCGCTGTGGATTAGTTCATTTCC |
| 5 | patA promoter | GATAGGGCAGAAGAGCATCC | GATAACGCGGTTGCAGAAGT |
| 6 | patA qRT–PCR | TCTTAGGCGCCCTCCTTACT | ATAGGCTGCGAGGACAAC |
| 7 | patB qRT–PCR | AGAAATGTGACGCTGGCTCT | TTCTGCTGGAGGTTGGTGT |
| 8 | guaA qRT–PCR | GCGCTTCGTCAGAATAAACC | AGTCCTTGCCAGTGACCTTC |
| 9 | spr1886 qRT–PCR | GGATTGGGAATCGTTTAGGG | AGAATCCAGTCCAGCGAAAG |
| 10 | hexA qRT–PCR | TGTCTAGTGTGCCACGGATT | CGCTGCGCTAATCAAACTCT |
| 11 | PBAV1K-gfp82 cloning site | TAGTATCGACGGAGCCGATT | TGTGCCCATTAACATCACCA |
| DNA oligonucleotide | |||
| WT patA upstream region | taagaattcaaccaagactcactagttaatctagctgtatcaaggagacttctttgacaattctccacttttttgcta gaataacatcacacaaacagaatgaaaaggagctgacgcattgtcgctcccttttgtctattttttctagaaag | ||
Bold and underlined text represents restriction enzyme cleavage sites.