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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: Biochemistry. 2013 Apr 30;52(19):3242–3253. doi: 10.1021/bi3010889

Figure 8.

Figure 8

Filipin accumulation in NPC2-defficient fibroblasts treated with β-CD:Pluronic PRTx. Human NPC2 deficient fibroblasts were incubated with 25 μM monomeric β-CD or PRTx yielding 25 μM β-CD and were fixed and stained with 0.05 mg/mL filipin at varying time points. (A) Representative images reveal stimulation of cholesterol efflux from npc2−/− fibroblasts by β-CD and two PRTx compounds over time. (B) Filipin accumulation was determined as the ratio of filipin stain area to total cell area. Results are expressed relative to control untreated cells, and are represented as mean ± SE (n = 3). (C) Viability of npc2−/− fibroblasts was determined by Trypan Blue staining 6 h after administration of each β-CD:Pluronic PRTx. Results are expressed relative to control untreated cells.