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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: Biochemistry. 2013 Apr 30;52(19):3242–3253. doi: 10.1021/bi3010889

Figure 9.

Figure 9

Fluorescein-labeled β-CD/F127 PRTx localizes to the LE/LY in npc2−/− fibroblasts. (A) Representative image of fluorescein localization in NPC2 deficient fibroblasts following a 1 h incubation period with labeled β-CD:F127 PRTx. (B) Lysotracker Blue staining identifies LE/LY compartments. (C) Fluorescein fluorescence overlays with Lysotracker Blue, indicating presence of labeled β-CD:F127 PRTx in the LE/LY compartment. (D) Intensity plot for each stain throughout an image section as indicated by the white arrow in panel C. Overlap in the localization of peak intensities is shown.