Fig 5. Ara-LAM induced the MHC-II expression in the membrane of L. donovani infected cells and helped in parasite killing.
(A) Peritoneal macrophages (2x106cells) isolated from BALB/c mice were pre-treated with Ara-LAM (3μg/ml) for 3hrs, followed by Leishmania donovani challenge for 24hrs. Both rIFN-γ (20ng/ml) stimulated (for 24hrs) and non-stimulated cells were then stained with anti-MHCII-FITC antibody and analyzed by Flow cytometry for MHC-II. Data are from one of three representative experiments. (B) In a similar set of experiment, the macrophages were cultured in cover slips, treated with Ara-LAM for 3 hrs followed by Leishmania infection and rIFN-γ stimulation (20ng/ml). After 24hrs of incubation intracellular parasite number were assessed as described in methods. Data represent means ± SD for three sets of experiments. ***P <.001 for the comparison with infected macrophages.