Fig 6. Induction of IFN-γ and IL-10 secreting CD4⁺ T cells by Ara-LAM in Leishmania-infected mice.

Twenty eight days after infection, different groups of mice were sacrificed, splenocytes and hepatocytes were isolated and stimulated with soluble leishmanial antigen (SLA) for 48hrs. Before harvesting, cells were incubated with brefeldin A (10 mg/mL) for 4 hrs. CD4⁺ T cells were purified by Magnetic Associated Cell Sorter (see Materials and Methods), permeabilized (0.1% saponin) and stained with anti-mouse IFN-γ-FITC (A) and anti-mouse IL-10-PE (B) antibodies and were analyzed by flow cytometry. Data are from one of three experiments conducted in the same way with similar results.