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. 2015 Feb 6;10(2):e0117247. doi: 10.1371/journal.pone.0117247

Table 1. Sequences of the PCR primers.

Gene Sequences of primers
IL-12p40 Forward 5’-ATTGACTGGCGTTGGAAGCA-3’
Reverse 5’-TGCGCTGGATTCGAACAAAGA-3’
IFN-γ Forward 5’-AGCTCTTCCTCATGGCTGTTTC-3’
Reverse 5’-TGTTGCTGATGGCCTGATTGT-3’
IL-10 Forward 5’-ACTTGGGTTGCCAAGCCTTAT-3’
Reverse 5’-ATCACTCTTCACCTGCTCCACT-3’
iNOS Forward 5’-CCTGACTGAAGCACTTTGGGTG-3'
Reverse 5’-TACAGGAAAGGCCCAAGCCAT-3'
IRF-1 Forward 5’- CAGAGGAAAGAGAGAAAGTCC-3’
Reverse 5’- CACACGGTGACAGTGCTGG-3’
IRF-2 Forward 5’- CAGTTGAGTCATCTTTGGGGC-3’
Reverse 5’- TGGTCATCACTCTCAGTGG-3’
IRF-3 Forward 5’- TACGTGAGGCATGTGCTGA-3’
Reverse 5’- AGTGGGTGGCTGTTGGAAAT-3’
IRF-4 Forward 5’- GTGACTGTGCCCTGGCTTAT-3'
Reverse 5’- TGGACATGATCTGGGCAACC-3'
IRF-5 Forward 5’- AATACCCCACCACCTTTTGA-3’
Reverse 5’- TTGAGATCCGGGTTTGAGAT-3’
IRF-6 Forward 5’- GATGTACGATGGCACCAAGG-3’
Reverse 5’- ACCGTTGATGTTCAGGAAGG-3’
IRF-7 Forward 5’- TGCAGAAGGTGGTGGGACA-3’
Reverse 5’- TGCTATCCAGGGAAGACACA-3’
IRF-8 Forward 5’-AACTGTGCTCTGGGCTCATC-3'
Reverse 5’- CCTCCGGGAAGTGTCCCTTA-3'
IFN-γRα promoter Forward 5’- GCTGCCAGTCTTCTAGTGGGA-3’
Reverse 5’- TCCTTGAGAAGTGCTGCTGGG-3’
IL-12p40 promoter Forward 5’-AAATTCCCCCAGAATGTTTTGACAC-3’
Reverse 5’-CGAGCTGCCTGGTCTGATGT-3’
IL-10 promoter Forward 5’-GACCTGGGAGTGCGTGAATGG-3’
Reverse 5’-AGCGCTAAAGAACTGGTCGGAATG-3’
GAPDH Forward 5’-GTTGTCTCCTGCGACTTCAACA-3’
Reverse 5’-TCTCTTGCTCAGTGTCCTTGCT-3’
β-actin Forward 5’-GATGATATCGCCGCGCTCGT-3’
Reverse 5’-GTAGATGGGCACAGTGTGGGTG-3’